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3.12. What is the easiest way to capture fluorescent and brightfiled image without acquiring each image and then merging?

A more efficient way to perform this action is to Acquire two channels but use Manual Intervention in the Bright field light path.

Steps to take:

-  Double click on the light path manager button

-  The light Path Properties dialog opens

-  If the light path requires changes in the set up that is needed to be done manually, check the box for Requires Manual Intervention

A message will be shown each time the light path is called prompting you to make the manual change.