Home → Volocity → General → What is the easiest way to capture fluorescent and brightfiled image without acquiring each image and then merging?
A more efficient way to perform this action is to Acquire two channels but use Manual Intervention in the Bright field light path.
Steps to take:
- Double click on the light path manager button
- The light Path Properties dialog opens
- If the light path requires changes in the set up that is needed to be done manually, check the box for Requires Manual Intervention
A message will be shown each time the light path is called prompting you to make the manual change.