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3.13. What is the difference between Z step and µm/pixel (z) in the Properties

Z step (µm) is the physical step used to capture the original images, changing this will not effect how the data is represented in the image, it is purely for reference purposes.

µm/pixel (Z) is the Z calibration, the optical step corrected for spherical aberration. It may be the same as the physical step but can be changed to correct for spherical aberration.